• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    This invention relates to biochemistry. The purpose of the invention is to increase the sensitivity of the agent. Filter paper is impregnated with a medium containing aqueous buffer from borax and hydrochloric acid, the main reagent and additional. Then the filter paper is immersed in a bottle containing 200 white blood cells / µl. The sensitivity of the agent is the higher, the more intense the coloring. 7 tab.
    公开号:SU1466663A3
    申请号:SU813278845
    申请日:1981-05-08
    公开日:1989-03-15
    发明作者:Бергер Дитер；Фрей Гюнтер；Кнаппе Вольфганг-Райнхольд；Кур Манфред；Риттерсдорф Вальтер；Вернер ВОЛЬФГАНГ
    申请人:Берингер Маннхайм Гмбх (Фирма)；
    IPC主号:
    专利说明:

    I 7I 8
    axes,
    about- "
    / n o (yN- n
    OSI,
    n
    n
    H

    Alani n Toluensulfonyl.
    DOS
    Bf.
    115
    OSI,
    n
    Bf4
    129
    Table 2
    L-ala
    Shh
    201
    Example 1. Filter paper is passed through with an agent containing 99-99.9 mol.% Aqueous buffer from borax and hydrochloric acid, 0.05 mol.% Having a pH, the reagent specified in Table 4 and 0.05 mol.%, Indicated at
    T a b l n c a 3
    table.4 additional reagent, followed by the substance at 60 C.
    Cooked this way
    filter paper is immersed for 3 minutes in urine containing 200 leukocytes / µl, after which it is determined visually11
    146666312
    are the resulting color. The coloring is more sensitive. The effectiveness of the means is higher than the intensities are given in Table.
    Active substances
    Additional reagent
    19 19
    nineteen
    nineteen
    XIII
    XIII
    XIII
    XIII
    Xxvii
    Xxvii
    Xxvii
    XXVIII
    XXIX
    XVI
    XVII
    XVIII
    nineteen
    The proposed tool
    Red-brown Red-brown
    gray-blue
    red
    light red
    purple
    lilova
    red and purple
    purple
    gray-blue
    lilova
    lilova
    light red
    light red
    red-violet
    red and purple
    According to the prototype
    Reddish
    Reddish
    Slightly reddish brown
    Bluish gray
    Known remedy Sinevata
    Comparison of the color data given in Table 4 indicates that the proposed agent shows a higher sensitivity than the known ones.
    with
    Example 2. 1 ml. Means holding 98 mol.% Aqueous phosphate buffer with a pH of 7.0, 1 mol.% Specified in the table.5 reagent and 1 mol.% Specified in the table, 5 additional reagent, mixed with 1 ml urine containing 100 leukocytes / µl. Three minutes after mixing, the extinction at 600 nm was determined with a photometer. The results of the experiments are given in table.5
    T a
    b l and c a 4
    Color after 3 min
    Table3
    45
    50
    gg
    facilities
    2.4
    2.5
    2.1
    131466663
    Continuation of table.5
    14
    Continuation of table.5
    The proposed tool
    . The comparison of the data in Table B indicates that, with a high content of active substances, known agents are inferior in sensitivity to the proposed composition
    PRI me R 4. The process is carried out analogously to example 2, except that use a tool containing 1 mol.% Reagent, 1.1 mol.% Additional reagent 1 or I and 98 mol.% The following aqueous buffer:
    a) sodium borate / hydrochloric acid with pH 8;
    b) sodium borate / boric acid with pH 8;
    c) sodium borate with pH 6;
    d) potassium phosphate with pH 6;
    e) potassium phosphate with pH 8;
    e) potassium phosphate with pH 10;
    T a
    b l and c a 6
    h) Tris buffer with pH 8. The results of the experiments are given in
    .bl.7.t a b l and c a 7
    权利要求:
    Claims (1)
    [1]
    Invention Formula
    Means for determining leukocytes in the urine, containing a reagent based on the diazonium salt of benzene derivatives and a buffer substance, characterized in that, in order to increase the sensitivity of the agent, it contains as a diazonium salt a compound of the general formula T
    X
    where is r,
    BJ, RS
    - hydrogen, chlorine, bromine.
    C, -Su-alkyl, Su-alkoxy, methyl mercapto group, N-morpholino, K-Thiomorpholino, N-pyrShIidino, if optionally substituted with methyl, N-piperazino, N-pyperdino;
    R is hydrogen, C -C-apkil, methoxy, bromine;
    R- hydrogen, fluorine, chlorine, bromine
    hydroxyl, C, -C-alkyl, C-Su-alkoxyl, methylamino group, C, -C5 - Dialkylamino, optionally substituted by methyl or methoxy phenyl group, phenoxy group, N-morpholino, N-thiomorpholino, N-pyrrolidino group, if necessary substituted with methyl, N-piperazino, N-piperidino, methyl mercapto;



    R.
    - hydrogen, methyl, methoxy.
    bromine;
    Rg is hydrogen, C) -C5 is alkyl, C-Cp-alkoxy, methyl mercapto, bromine and chlorine;
    X - tetrafluoroborate, perchlorate and carbon tetrachloride; IV as an additional reactin contains a compound of general formula II
    66663
    where r
    one
    ten
    15
    jo 25
    - hydrogen, chlorine, bromine, meth- 6
    OKOA, pentyl;
    R is hydrogen, bromine, kil, methoxy, HjiTpo, acetylamino, annelated benzene ring;
    Rg is hydrogen, bromine, methyl, ethoxy, benzyl, annelated benzene ring;
    R is hydrogen, bromine, methyl,
    phenyl;
    A - alanyl, L-apanil-apanil, L-apanil-alanyl-alanyl, glycyl, valyl, L-leucyl, phenylalanil;
    B is benzoyl, toluenesulfonyl, acetyl, benzyloxycarbonyl, acetimide-benzenesulfonyl, and methoxytyosyl, or contains a compound of the general form OA-B 11
    tel K
    where is hydrogen, C-C-alkyl, C.-Sdalkoxy, methylamino and dimethylamino;
    R ,, is hydrogen, ethyl, methyl, methoxy, dimethylamino, diethylamino;
    R, is hydrogen, methyl, C -Cs-anK- hydroxy, C -C-alkylamino, dipropylamino;
      hydrogen, methyl, ethyl, methoxy, and the same two adjacent substituents
    R
    ti
    R
    year
    and R ,, is annelated benzene ring;
    A - alanyl, L-alanyl-alanyl, b-alanyl-alanyl-apanyl, valyl, phenylalanyl, leucyl, glycyl;
    (c) toluenesulfonyl, acyl, benzoyl, benzyloxycarbonyl, acetamidobenzenesulfonyl, benzoylcarbonyl, and methoxytyosyl,
    at the following ratios of components, mol,%:
    The compound of General formula G0.05-25
    The compound of General formula II or the compound of General formula III 0.05-25 Buffer substance with a pH of 6-10 The rest
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    同族专利:
    公开号 | 公开日
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    AR228452A1|1983-03-15|
    HU187334B|1985-12-28|
    PL131123B1|1984-10-31|
    MY8600605A|1986-12-31|
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    CS223999B2|1983-11-25|
    ES502034A0|1982-03-01|
    US4551428A|1985-11-05|
    PL231002A1|1981-12-23|
    DE3017721A1|1981-11-26|
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    FI811418L|1981-11-10|
    FI77692B|1988-12-30|
    CA1186201A|1985-04-30|
    JPS578798A|1982-01-18|
    JPS61199800A|1986-09-04|
    ZA812961B|1982-05-26|
    US4749648A|1988-06-07|
    ES8202792A1|1982-03-01|
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    YU112483A|1989-10-31|
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    SG38786G|1989-09-01|
    JPH0243480B2|1990-09-28|
    DK169974B1|1995-04-18|
    HK82586A|1986-11-07|
    YU45887B|1992-09-07|
    FI77692C|1989-04-10|
    DD158559A5|1983-01-19|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    GB1128371A|1965-10-04|1968-09-25|Miles Lab|Diagnostic composition|
    US3585001A|1969-02-17|1971-06-15|Miles Lab|Stabilized test device and process for detecting couplable compounds|
    US3715325A|1970-10-02|1973-02-06|Miles Lab|Insoluble polymeric diazonium salt chromogen|
    US3905872A|1973-12-14|1975-09-16|American Cyanamid Co|Alkaline phosphatase test material|
    DE2531539A1|1975-07-15|1977-01-20|Riedel De Haen Ag|Diazonium salt indicators - for detecting coupling components in liqs. for diagnostic purposes|
    DE2836644A1|1978-08-22|1980-03-06|Boehringer Mannheim Gmbh|DIAGNOSTIC AGENT FOR DETECTING LEUCOCYTES IN BODY LIQUIDS AND CHROMOGENES SUITABLE FOR THIS|
    DE2854987A1|1978-12-20|1980-06-26|Boehringer Mannheim Gmbh|DIAGNOSTIC AGENTS FOR DETECTING PROTEOLYTIC ENZYMS AND CHROMOGENS SUITABLE FOR THIS|
    DE2905531A1|1979-02-14|1981-01-08|Boehringer Mannheim Gmbh|DIAGNOSTIC AGENT FOR DETECTING LEUCOCYTES IN BODY LIQUIDS|
    DE2936543A1|1979-09-10|1981-04-09|Behringwerke Ag, 3550 Marburg|CHROMOGENIC COMPOUNDS|
    DE3017721A1|1980-05-09|1981-11-26|Boehringer Mannheim Gmbh, 6800 Mannheim|AGENT FOR DETECTING ESTEROLYTIC AND / OR PROTEOLYTIC ENZYME AND SUBSTRATES SUITABLE FOR THIS|DE3017721A1|1980-05-09|1981-11-26|Boehringer Mannheim Gmbh, 6800 Mannheim|AGENT FOR DETECTING ESTEROLYTIC AND / OR PROTEOLYTIC ENZYME AND SUBSTRATES SUITABLE FOR THIS|
    US4499185A|1982-05-17|1985-02-12|Miles Laboratories, Inc.|Test for esterase activity in a liquid sample|
    DE3309544A1|1983-03-17|1984-09-20|Macherey-Nagel & Co Chemikalienhandel, 5160 Düren|TEST STRIP FOR DETECTING LEUCOCYTES IN THE URIN|
    DE3329394A1|1983-08-13|1985-02-28|Bayer Ag, 5090 Leverkusen|CHROMOGENIC AND FLUOROGENEIC CARBONIC ACID ESTERS, METHOD FOR THE PRODUCTION THEREOF AND METHODS FOR DETECTING AND DETERMINING HYDROLASES|
    EP0146348B1|1983-12-14|1991-07-31|The Upjohn Company|Substituted naphthalenes, indoles, benzofurans and benzothiophenes as lipoxygenase inhibitors|
    US4637979A|1984-04-06|1987-01-20|Miles Laboratories, Inc.|Composition and test device for determining the presence of leukocytes containing a zwitterion coupling agent|
    DE3413077A1|1984-04-06|1985-10-17|Miles Laboratories, Inc., Elkhart, Ind.|CHROMOGENEIC AMINO ACID AND PEPTIDESTERS, METHOD FOR THE PRODUCTION THEREOF, USE OF THESE COMPOUNDS IN ANALYZING METHODS AND MEANS FOR DETECTING ESTEROLYTIC AND / OR PROTEOLYTIC ENZYMES|
    DE3413119A1|1984-04-06|1985-10-24|Miles Laboratories, Inc., Elkhart, Ind.|ANALYSIS METHOD AND MEANS FOR DETECTING ESTEROLYTIC AND / OR PROTEOLYTIC ENZYMS|
    DE3413078A1|1984-04-06|1985-10-24|Miles Laboratories, Inc., Elkhart, Ind.|CHROMOGENEIC AMINO ACID AND PEPTIDESTERS, METHOD FOR THE PRODUCTION THEREOF, USE OF THESE COMPOUNDS IN ANALYZING METHODS AND MEANS FOR DETECTING ESTEROLYTIC AND / OR PROTEOLYTIC ENZYMES|
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    US4777267A|1986-02-07|1988-10-11|Kanebo Ltd.|1,3-dioxol-2-one derivatives|
    US4677075A|1986-05-05|1987-06-30|Louderback Allan Lee|Urobilinogen control|
    DE3813503A1|1988-04-22|1989-11-02|Boehringer Mannheim Gmbh|CARRIER-TIED MULTI-COMPONENT DETECTION SYSTEM FOR THE COLORIMETRIC DETERMINATION OF ESTEROLYTIC AND / AND PROTEOLYTICALLY ACTIVE INGREDIENTS OF BODY LIQUIDS|
    US5084324A|1989-08-25|1992-01-28|W. R. Grace & Co.-Conn.|Micro-bubble laminate with perforated substrate|
    US4950354A|1989-08-25|1990-08-21|W. R. Grace & Co.-Conn.|Method of making a micro-bubble laminate|
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    CA2161574A1|1994-11-15|1996-05-16|James Noffsinger|Methodology for colorimetrically determining the concentration of white blood cells in a biological fluid|
    US6348324B1|1999-01-21|2002-02-19|Hypoguard America Limited|Composition and device for detecting leukocytes in urine|
    US6528652B1|1999-01-21|2003-03-04|Chronimed|Composition and device for detecting leukocytes in urine|
    US6203496B1|1999-08-12|2001-03-20|Michael R. Gael|Apparatus with reagents for detection of medical conditions|
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    US6955921B2|2001-04-30|2005-10-18|Bayer Corporation|Trypsin substrate and diagnostic device, and method of using same|
    US6709868B2|2002-05-20|2004-03-23|Portascience Inc.|Method and apparatus for measuring white blood cell count|
    US7727206B2|2005-12-27|2010-06-01|Gorres Geoffrey H|Device for monitoring a patient for a urinary tract infection|
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    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    DE19803017721|DE3017721A1|1980-05-09|1980-05-09|AGENT FOR DETECTING ESTEROLYTIC AND / OR PROTEOLYTIC ENZYME AND SUBSTRATES SUITABLE FOR THIS|LV930449A| LV5342A3|1980-05-09|1993-06-01|Composition of urine for leukocyte detection|
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